ICUMSA Method GS1/2/3-4 (1998)

The Determination of Glucose and Fructose in Raw and White Sugars Using High Perfomance Anion-Exchange Chromatography (HPAEC) - Official

ICUMSA Method GS1/2/3-4 (1998)
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Abstract:
The glucose and fructose content in raw and white sugars is determined using High Performance Anion-Exchange Chromatography. The method is suitable for contents of glucose and fructose between 0.005 g/100 g and 0.15 g/100 g in the sample. Sugars are separated using a pellicular anion exchange column and detected using a pulsed amperometric detector (PAD). Comparison with a lactose standard solution provides identification and quantification. For raw sugars with glucose or fructose levels between 0.08 g/100 g and 0.23 g/100 g the absolute difference between two results obtained under repeatability conditions should not be greater than 0.02 g/100 g and under reproducibility conditions not greater than 0.03 g/100 g. For white sugars with glucose or fructose levels between 0.004 g/100 g and 0.017 g/100 g the absolute difference between two results obtained under repeatability conditions should not be greater than 0.002 g/100 g. For white sugars with glucose levels between 0.004 g/100 g and 0.014 g/100 g the absolute difference between two results obtained under reproducibility conditions should not be greater than 0.006 g/100 g. For white sugars with fructose levels between 0.004 g/100 g and 0.017 g/100 g the absolute difference between two results obtained under reproducibility conditions should not be greater than 0.008 g/100 g.

Online Access to ICUMSA Method GS1/2/3-4 (1998)

Access to the online Version of ICUMSA Method GS1/2/3-4 (1998) can be obtained from the Sugar Industry Website, operated by Bartens. The online access for two years costs EUR30 (+ 19 % VAT for German and EU citizens without EU VAT No.).