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ICUMSA Method GS 2/3-52 (2017)
ß-Glucuronidase-positive Escherichia coli and ß-Galactosidase-positive Coliform Bacteria in Sugar

1 Scope and Field of Application

This method is used for the determination and enumeration of Escherichia coli (E. coli) and coliform bacteria in sugar products by means of membrane filtration of dissolved sugar/sugar products and incubation of the membrane on agar plates or nutrient pads containing special chromogenic or fluorogenic substrates. These are hydrolysed by either coliform bacteria or E. coli and the colonies appear in different colours or the fluorescence of a colony can be visualised by exposure to UV-light at 366 nm. Chromogenic substrates which are hydrolysed by coliform bacteria are those that contain a dye bound to galactose (e.g. 6-chloro-3-indolyl-β-D-galactoside (Salmon-Gal) or 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) [1]). Chromogenic substrates which are hydrolysed by E. coli but not coliform bacteria are those containing a β-D-glucuronide (e.g. 5-bromo-4-chloro-3-indolyl-β-d-glucuronide (X-Gluc) [2] or 4-methylumbelliferyl-β-D-glucuronide [3]).

Online Access to ICUMSA Method GS 2/3-52 (2017)

Access to the online Version of ICUMSA Method ICUMSA Method GS 2/3-52 (2017) can be obtained from the Bartens Website. The online access for two years costs EUR25 (+ 19 % VAT for German and EU citicens without EU VAT No.). You will receive your access code within 48 hours after receipt of payment.