Abstract: The method is used to determine the sulphite content for refined sugars by an enzymatic procedure. Where a choice exists for white sugar then method GS2-33 is preferred.
The enzymatic procedure results in the formation of hydrogen peroxide which is reduced by NADH peroxidase; the resultant absorption decrease is measured using a spectrophotometer.
The method has Official Status for refined sugars except for brown sugars for which it has Tentative Status.
The absolute difference between two results obtained under repeatability conditions for white sugars between 4.02 mg/kg and 23.84 mg/kg sulphite should be in the range of 1.17 mg/kg to 6.49 mg/kg and under reproducibility conditions in the range of 1.74 mg/kg to 14.58 mg/kg.
Online Access to ICUMSA Method GS2/3-35 (2011)
Access to the online Version of ICUMSA Method GS2/3-35 (2011) can be obtained from the Sugar Industry Website, operated by Bartens. The online access for two years costs EUR30 (+ 19 % VAT for German and EU citizens without EU VAT No.).